TRANSFORMATION IN YEAST: DEVELOPMENT OF A HYBRID CLON- ING VECTOR AND ISOLATION OF THE CAN! GENE (Saccharomyces cerevisiae, plasmid vector; arginine permease)
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چکیده
We have constructed a plasmid, YEpl3, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2/~ circle. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEpl3.
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تاریخ انتشار 2002